Solving problems of worsening phytosanitary situation of crops, requires using methods of microbiological protection of crops. Isolation, identification and determination of biological agents (viruses, bacteria, fungi, actinomycetes) were used to develop biological products to biological combat of pests and disease pathogens. Were developed and approved four new biological means: Virin-HSP - to combat Helicoverpa armigera, Paurin - to combat Agrobacterium tumefaciens, Trichodermin SC - to combat pathogens sunflower, soybean, grape-vine , Gliocladin SC - to combat white rot in sunflower (Sclerotinia sclerotiorum), Fusarium spp. on soya, and Botrytis cinerea on grape. .Ecological means ensures high degree of combating pests, economic and environmental efficiency and used in conventional and organic farming systems.