Silver nanoparticles (AgNPs) are intensely studied especially because silver is a noble metal with excellent antibacterial, antimicrobial and antifungal properties and silver-based compounds are cheaper than their corresponding gold-based ones. In the last decade, the scientific studies regarding the eco-friendly synthesis of AgNPs have exponentially grown and plant-based AgNPs are especially studied with good and reproducible results. The use of different plants or plant parts for the synthesis of AgNPs offers a wide range of advantages and experimental results prove that this method is biocompatible, cost-effective and can be applied to large-scale synthesis. Elderflower (Sambucus nigra L.) is recognized all over the world for its antiseptic and anti-inflammatory properties and is therefore used to treat colds, sinus infections and other respiratory diseases. This paper presents the green synthesis of AgNPs using Elderflower aqueous extract in comparison with chemically obtained AgNPs via sodium-citrate. The first evidence that AgNPs are formed is visually noticed by a change of color, from light brown (Elderflower aqueous extract) to yellow-grey (Elderflower-AhNPs). The formation of AgNPs is confirmed by different spectral techniques: UV-Vis (recorded at several time intervals), FTIR, SEM, TEM and the nanometer size of the prepared particles is studied using DLS measurements. In conclusion, a new method to prepare silver nanoparticles by means of green chemistry was achieved by using the Elderflower aqueous extract. The fact that AgNPs are obtained is confirmed by different physical.