The microbial-derived bioproducts (fungicides, herbicides, insecticides) are used in agriculture for stimulating plant growth, development and defense against pathogens. Increase of plant productivity and improvement of crop quality is one of the main tasks of biological and agricultural sciences. The microorganisms – producers of bioactive substances play an important role in achieving this goal. The micromycetes as Trihoderma, Penicillium etc. are among the widely used producers of biofungicides, combating different plant diseases and stimulating plant growth. The aim of this research was to study the antifungal and phytostimulating activity of exometabolites of the strains Penicillium sp. 32 and Penicillium sp.62. The antigfungal activity was evaluated against 10 phytopathogenic fungi: Aspergillus niger; Alternariaalternata; Botrytis cinerea; Sclerotiniasclerotiorum; Stemphiliumbotryosum; Rhizoctoniasolani; Penicilliumexpansum; Fusarium solani; Fusarium oxysporum; Fusarium gibbosum, using the method of agar blocks. Phytostimulating properties of studied exometabolites (EM) were tested on wheat seeds. The results showed that both strains of Penicillium inhibited the growth of mentioned phytopathogenic agents. The efficacy of exometabolites of Penicillium sp.62 was evaluated in laboratory conditions by treating infected by Fusarium oxysporum seeds. The antifungal activity of solution of EM of Penicillium sp. 62 in a concentration of 100% and 1: 100 (EM : distilled water) was found to be 4 times higher compared to the control sample. Also EM solution at a concentration of 1: 100 showed a positive effect on the growth and development of seedlings root. According to obtained results, we can conclude that the strains Penicillium sp. 32 and Penicillium sp.62 show a high antifungal action against a broad spectrum of phytopathogenic fungi and could serve as potential producers of biofungicides. The exometabolites of Penicillium sp. 62 of low concentrations proved its efficacy against the pathogen fungi Fusarium oxysporum and could be used for the pre-treatment of seeds before sowing, as a biological method to combat fusariosis diseases and to stimulate seedlings growth.