Introduction: Synthesis and characterization of nanoparticles is an active area of research because it offers solutions to environmental and technological challenges in different fields, such as medicine, biology, catalysis, electronics and energy. Most of the current techniques are expensive, high energy consuming and use toxic and dangerous chemicals, responsible for damaging the environment and biological systems. This problem can be overcome by the use of nanobiotechnology, in which the metal nanoparticles are generated using various biological agents, such as extracts from plants, bacteria, fungi, algae. The purpose of this study was to synthesize silver nanoparticles (AgNPs) using Tilia cordata (linden) flowers extracts and to assess their antimicrobial activity. Materials and methods: The synthesis of AgNPs was performed by exposure of linden flowers extracts to silver nitrate aqueous solution, in different conditions. The optimization of various parameters was investigated, such as pH, concentration of silver salt, different ratio of extract and silver salt, temperature and stirring time. AgNPs were characterized by UV-Vis spectroscopy, FTIR, SEM. Antimicrobial activity of AgNPs was performed by agar diffusion method on Mueller-Hinton medium, recording diameters of zones of inhibition of microbial growth, against Gram positive bacteria, Gram negative bacteria and fungi. Results and conclusions: During the synthesis process, a color change of the solution was observed from yellow through brown-yellow, brown, reddish to dark brown. UV-Vis spectroscopy analysis showed the Surface Plasmon Resonance band corresponding AgNPs, centered at 430-460 nm. The maximum absorption increased with concentration of silver salt, stirring time and temperature. The optimum reaction pH was 6. FTIR analysis confirmed the reduction of Ag+ and the formation of AgNPs, the extract through its secondary metabolites being used as reducing agent and capping agent. SEM images showed a high density of AgNPs synthesized. The synthetized AgNPs were found to be effective against some of the tested microorganisms. In conclusion, a simple, fast, cost-effective and ecofriendly method has been developed to obtain AgNPs with antimicrobial activities, using extracts linden flowers.