Over the last two decades the adhesive characteristics of bacteria and other cells as well as their surface components have assumed increasing significance in microbial ecology. Adhesion to epithelial gut cells is an important prerequisite for colonization by lactobacilli in the gastrointestinal tract; it promotes the formation of a barrier to prevent colonization of pathogenic microorganisms. Determination of adhesion activity by Western - blot analysis with mucin, labeled with horseradish peroxidase (HRP) as the target ligand adhesion allow to monitor stages, purity and molecular weight of adhesive protein. The purpose of this study was to determine the adhesive activity of lactic acid bacteria by Western - blot analysis. Objects of study were cultures of lactic acid bacteria (LAB) isolated from a variety of meat and dairy foods of home and industrial production. A well-known method of identification of lactic acid bacteria is mainly focused on the use of the determinant «Bergey Manual of Systematic Bacteriology». Cell viability was determined by serial dilutions followed by spreading on solid media. To determine the nucleotide sequence of 16S rDNA, DNA was extracted by Kate Wilson, and the PCR reaction was performed with universal primers 8f -5 ‘AgAgTTTgATCCTggCTCAg- 3 and 806R- 5 ‘ggACTACCAgggTATCTAAT in a total volume of 30 microliters. Purification of PCR products was carried out using an enzymatic method, ExonucleaseI (Fermentas) and alkaline Phosphatase (Shrimp Alkaline Phosphatase, Fermentas). The sequencing reactions were performed using Big Dye® Terminator v3.1 Cycle Sequencing Kit (Applide Biosystems) according to the manufacturer’s instructions, followed by separation of the fragments on an automated genetic analyzer 3730xl DNA Analyzer (Applide Biosystems). Test phenotypes of autoaggregation for selected LAB strains obtained by the method of Kos B, Suskovic J, (2003). Western - blot analysis with mucin, labeled with horseradish peroxidase (HRP) was carried out on the general protocol of a Western blot (TE2 protocol producer tank transfer unit). According to the results of study the autoaggregation of the selected 25 LAB strains, we chosen strains with a maximum value of % autoaggregation. Highest % autoaggregation showed the strain Lactobacillus plantarum K2 isolated from koumiss, as well as Lactobacillus pentosus S9 (homemade sour cream), Lactobacillus paracasei tolerans P10 (kazi), Lactobacillus paracasei tolerans K4 (mare milk), Lactobacillus sakei S10 (homemade cream) and the lowest % autoaggregation showed strains: Lactobacillus pentosus S3 (homemade sour cream), Lactobacillus plantarum K1 (mare milk) and Lactobacillus delbruckii sub.lactis K5 (mare milk). In the first step of the Western blot was performed electrophoresis by polyacrylamide gel with sodium dodecyl sulfate (SDS-PAGE electrophoresis) to separate proteins. The strain with high autoaggregation % (Lactobacillus paracasei tolerans P10) was taken as a selected sample. In the next stage, we performed Western blot samples for transformation with mucin, labeled with horseradish peroxidase (HRP) as the target ligand adhesion. The result of a Western blot on nitrocellulose membrane was visible adhesive protein with a molecular weight of about 76,82 kDa, which indicates adhesive activity isolates and indicate the presence of the protein - adhesion. So the horseradish peroxidase labeled by gastric mucin associated with the selected experiment sample, showed a high % of autoaggregation. According to the literature there are multi-functional proteins with similar molecular weight.