The total phenols content and the antioxidant capacity of different varieties of apples cultivated in the Transylvania area
Închide
Articolul precedent
Articolul urmator
572 11
Ultima descărcare din IBN:
2023-09-20 00:04
SM ISO690:2012
BECZE, Anca, SIMEDRU, Dorina, ROMAN, Marius, DORDAI, Lucian. The total phenols content and the antioxidant capacity of different varieties of apples cultivated in the Transylvania area. In: Microbial Biotechnology, 12-13 octombrie 2016, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2016, Ediția 3, p. 90.
EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core
Microbial Biotechnology
Ediția 3, 2016
Conferința "Microbial Biotechnology"
Chișinău, Moldova, 12-13 octombrie 2016

The total phenols content and the antioxidant capacity of different varieties of apples cultivated in the Transylvania area


Pag. 90-90

Becze Anca, Simedru Dorina, Roman Marius, Dordai Lucian
 
Research Institute for Analytical Instrumentation, ICIA Cluj-Napoca
 
 
Disponibil în IBN: 14 martie 2019



Teza

From the ideal perspective, a balance between free radicals and antioxidant substances/ neutralizing mechanisms in the body should exist. When these mechanisms are overwhelmed by the free radicals, they cannot be effectively neutralized and begin to be accumulated in a process called oxidative stress. The results of long-term exposure to oxidative stress are degradation and genetic mutations at the cellular level, and as direct effects, premature aging, inflammation, particularly that of blood vessels and arteries, cardiovascular diseases, autoimmune and neurodegenerative diseases like Alzheimer and Parkinson. A diet rich in foodstuffs that have a high antioxidant capacity is important to ensure the free radicals neutralizing in the body. Twelve varieties of Malus pumila fruits (apples) were analyzed in order to determine the total phenol content and antioxidant capacity but also to establish a correlation between these values. For the determination of the antioxidant capacity expressed in equivalents of vitamin C, the Photochem instrument from Analytik Jena was used. The instrument uses the fast photochemical excitation of radical formation combined with sensitive luminometric detection. Kits for antioxidant capacity of water soluble substances from Analytik Jena where used. The kits contain a standard solution of vitamin C, stock solution that has a photo sensitizer and a detection reagent, a buffer solution and a diluting solution. A calibration curve of vitamin C was done in 4 points 0,5nM, 1 nM, 2 nM and 3 nM. The measurement was done in 2 steps: 1.Optical excitation of a photosensitizer substance S and subsequent generation of the superoxide anion radicals S + hν + O2 → [S*O2] → S•+ + O2• -; 2.Detection of the radicals (remaining after the reaction with antioxidants) by using a chemiluminogenic substance. For the determination of polyphenols, a modified version of the Folin-Ciocalteu method was used. The samples were measured at 765 nm using the Spectrophotometer UV/VIS Lambda 25 from Perkin Elmer. Measurements were compared to a calibration curve of Gallic acid (25, 50, 100, 250, 500 ppm), and the results were expressed as Gallic acid equivalents. The obtained antioxidant capacity values varied from 400 to 1400 mg/kg of Ascorbic Acid equivalents. The total polyphenols content varied between 400 to 1350 mg/kg Gallic Acid equivalents. A positive correlation of 0.862 was obtained between the antioxidant capacity and the total polyphenols content. The high values of the total polyphenols content and antioxidant capacity prove that apples are a great source of antioxidants that can be found all year long. The relative low positive correlation between the values proves that not only polyphenols ensure the antioxidant capacity of apples but also other compounds like vitamin C and various phytochemicals.