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SM ISO690:2012 GULYA, Aurelian, ZARICIUC, Elena, MITKEVICH, N., TSAPKOV, Victor I., KOTOVAYA, A., RUDIK, V.. Antimicrobial and antifungal effect of some biometal coordination compounds of 5-nitro-2-furaldehyde 4-(methoxyphenyl)thiosemicarbazones. In: Microbial Biotechnology, 12-13 octombrie 2016, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2016, Ediția 3, p. 71. |
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Microbial Biotechnology Ediția 3, 2016 |
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Conferința "Microbial Biotechnology" Chișinău, Moldova, 12-13 octombrie 2016 | |||||||
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Pag. 71-71 | |||||||
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5-Nitro-2-furaldehyde 4-(2-, 3-, and 4-methoxyphenyl)thiosemicarbazones (HL1–3) react with cobalt(II), nickel(II), copper(II), and zinc chlorides, nitrates and acetates in molar ratio 1:1 or 2:1 forming coordination compounds with composition M(HL1–3)2X2 (M = Co, Ni, Cu, Zn; X =Cl-, NO3 -) and M(L1–3)2 (M = Co, Ni, Cu, Zn) regardless of used molar ratio. The antimicrobial activity of these compounds was studied in vitro using the method of two-fold serial dilutions in liquid nutrient medium (meat infusion broth, pH = 7.0). The substances were dissolved in DMSO in concentration of 10 mg/mL. The next dilutions were made using meat infusion broth. The antimicrobial activity was tested on standard strains of gram-positive (Staphylococcus aureus ATCC 25923), gram-negative (Escherihia coli ATCC 25922, Klebsiella pneumoniae) bacteria and Candida albicans fungus. 18-hours agar culture of these microorganisms was used for bacterial inoculation. Inoculation dose of 250–500 ths. microorganisms per 1mL was determined by optical turbidity standard. Test tubes were agitated and placed in thermostat at 370C for 24h. The test tube with nutrient medium and microorganisms but without the studied substance was used as control sample. The tested substance was defined as bacteriostatic at respective concentration, if there were no signs of visible growth of microorganisms in the liquid nutrient medium. For detecting bactericidal activity of tested substance, microorganisms were reinoculated on a solid nutrient medium (meat infusion agar) and incubated. The absence of visible growth of microorganisms indicates the bactericidal activity of tested substances at this concentration. It was established that HL1–3 are practically inactive towards used strains of microorganisms. Meanwhile, the synthesized coordination compounds with HL1–3 as ligands show selective bacteriostatic and bactericidal activity towards both gram-positive and gram-negative bacteria in the range of concentrations 0,030–10,0 mg/mL. The nature of the central atom and substituents R1–3 of the corresponding azomethine (HL1–3) have the main influence on the minimum inhibitory and minimum bactericidal concentrations. The activity changes in the following way: Сu > Co ≥ Ni ≥ Zn and HL2 ≥ HL1 ≥ HL3. Staphylococcus aureus and Candida albicans are the most sensitive strains to this series of substances. |