Biological activity of S. Levoris CNMN-Ac-01 after long-term storage by subculturing and under mineral oil
Închide
Articolul precedent
Articolul urmator
639 7
Ultima descărcare din IBN:
2022-03-04 16:12
SM ISO690:2012
BYRSA, Maxim, CARAMAN, Maryana, BURTSEVA, Svetlana. Biological activity of S. Levoris CNMN-Ac-01 after long-term storage by subculturing and under mineral oil. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, p. 167. ISBN 978-9975-3178-8-7.
EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core
Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

Biological activity of S. Levoris CNMN-Ac-01 after long-term storage by subculturing and under mineral oil


Pag. 167-167

Byrsa Maxim1, Caraman Maryana2, Burtseva Svetlana1
 
1 Institute of Microbiology and Biotechnology ,
2 Scientific and practical institute of Biotechnologies in Animal Husbandry Veterinari Medicine
 
Disponibil în IBN: 21 februarie 2019



Teza

Preservation of vitality and biotechnological properties of different types of actinobacteria is associated with certain difficulties due to the high level of their genetic instability. Different storage methods have their advantages and disadvantages. The aim of the studies was to determine the effect of long-term storage (10 years) of strain S. levoris CNMN-Ac-01 by subculturing and under mineral oil. It was found that during storage process by both subculturing and under mineral oil, the cultural properties of the strain practically did not undergo significant changes: in the colour of the aerial mycelium – a typical pinkish-paler shade predominated, and for substrate mycelium – yellowish one predominated during growth on classical Czapek media with glucose, Gause, or oat agar. The amount of biomass after storage of the strain changed as follows: initially, when cultivated on a liquid complex medium M-I, the strain accumulated up to 6.29 ± 0.34 g/l. After long-term storage by subculturing, the quantity of biomass decreased to 5.08 ± 0.12 g/l, then in the variant under mineral oil - to 5.4 ± 0.2 g/l. The quantity of total lipids in biomass was initially 12.54%, after storage – 11.8% and 12.1%, respectively. Comparing the fractional composition of the total lipids in biomass after long-term storage by both methods, it was noted that qualitative and quantitative composition of the main lipid fractions remained unchanged: the number of basic physiologically important fractions as phospholipids was 13-14.47% and sterols 16.0-17.7% of the total amount of lipids, regardless of the storage method. The ability to depress the growth of phytopathogenic test microorganisms (bacteria and fungi) has changed as follows: the activity against Erwinia carotovora was by 10.6-11.3% lower for the strain stored by subculturing, than for the same strain stored under mineral oil. The activity against C. michiganensis remained unchanged: up to 19.0-20.0 mm diameter of the growth inhibition zones. Also, no changes were noted regarding the phytopathogenic fungi A. niger and A. alternata (the size of the inhibition zones - 10.0-13.0 mm). A slight decrease was noted in activity against B. cinerea (15.0 mm – zone of growth inhibition at the beginning of studies and 13.0-13.5 mm after the storage by both methods). Thus, the conducted studies showed that during long-term storage (10 years) of S. levoris CNMN-Ac-01 by subculturing or under mineral oil, its biosynthetic activity (accumulation of biomass during cultivation on nutrient media and its lipid content) and antimicrobial activity against some phytopathogens decreased.