Effects of iron oxide nanoparticles on antioxidant enzymes activities of pigmented yeasts
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EFREMOVA, Nadejda, BEŞLIU, Alina, USATÎI, Agafia. Effects of iron oxide nanoparticles on antioxidant enzymes activities of pigmented yeasts. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, p. 153. ISBN 978-9975-3178-8-7.
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Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

Effects of iron oxide nanoparticles on antioxidant enzymes activities of pigmented yeasts


Pag. 153-153

Efremova Nadejda, Beşliu Alina, Usatîi Agafia
 
Institutul de Microbiologie şi Biotehnologie
 
Disponibil în IBN: 21 februarie 2019



Teza

Over the past decades, nanoparticles (NPs) of metal oxides are widely used in different fields, such as medicine, food, pharmaceutical and cosmetic industries. Iron oxide nanoparticles have a large surface area, high reactivity, novel magnetic properties. Furthermore, when compared to other metallic nanoparticles, the iron oxide NPs are less expensive, harmless, biocompatible and less toxic. However, there is a need to study the biochemical mode of iron oxide NPs action. As a redox metal, Fe participates in Fenton and Haber–Weiss reactions and the formation of reactive oxygen species (ROS) and oxidative stress. By modification of oxidation states, iron oxide nanoparticles further activate species like hydrogen peroxide (H2O2) and superoxide (O2 -) to the highly reactive hydroxyl radical. The cellular defense system against toxicity induced by ROS includes superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidases (GPx). In this study, the effects of different concentrations from 0.5 to 30.0 mg/l of Fe3O4 NPs on antioxidant activities of pigmented yeast strain Rhodotorula gracilis CNMN-Y-30 were investigated. Commercially available Fe3O4 nanopowder, (Sigma-Aldrich; particle size 50-100 nm) was used in the preparation of experimental solutions. Research was effectuated on the solid fermentation medium YPD specific for yeasts. Regarding the study of superoxide dismutase activity in yeast Rhodotorula gracilis CNMNY- 30 under the influence of Fe3O4 (50-100 nm) NPs, the significant increase was recorded with the first used concentration of 0.5 mg/l. The maximum SOD activity compared to control (by 90%) was achieved by the introduction of iron nanoparticles at concentration of 10.0 mg/l. Subsequent increases of concentrations contributed to a slight decrease of SOD activity. At the same time, the enzyme values prevailed control values by 78-37%. According to the results, Fe3O4 (50-100 nm) NPs influenced the activity of other antioxidant enzyme - catalase. The adaptive reaction response of Rhodotorula gracilis CNMN-Y-30 strain to the introduction of Fe3O4 nanoparticles (50-100 nm) was expressed by increasing the activity of catalase at concentrations of 0.5 ... 5.0 mg/L by 25 - 35%. The utilization of concentrations up to 30 mg/L contributed to the decrease with 5-50% compared to control. This can be explained by the excessive formation of reactive hydroxyl radical at high concentration of NPs. These results suggest that iron nanoparticles might exert an influence on the level of expression of SOD and CAT activity. Although, there are many antioxidant defense actions against oxidative stress, SODs constitute a significant mechanism among others. This study reports that Fe3O4 NPs induced oxidative stress, as indicated by a significant increase of superoxide dismutase and catalase enzyme activities in pigmented yeasts. These studies suggest that iron oxide nanoparticles contributed to ROS formation, which alter the protein metabolism via reactive oxygen species formation.