Influence of nanoparticles of Cu, Co, and ZnO on micromycetes
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TIMUȘ, Ion, SYRBU, T., MASLOBROD, Sergey, MIRGOROD, Yu., BORSCH, Nikolay, BORODINA, Valentina. Influence of nanoparticles of Cu, Co, and ZnO on micromycetes. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, p. 150. ISBN 978-9975-3178-8-7.
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Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

Influence of nanoparticles of Cu, Co, and ZnO on micromycetes


Pag. 150-150

Timuș Ion1, Syrbu T.1, Maslobrod Sergey2, Mirgorod Yu.3, Borsch Nikolay3, Borodina Valentina3
 
1 Institute of Microbiology and Biotechnology ,
2 Institute of Genetics, Physiology and Plant Protection,
3 Southwest State University
 
Disponibil în IBN: 21 februarie 2019



Teza

Nanoparticles are used successfully in various fields due to their very small size and outstanding physicochemical properties. Inorganic nanoparticles are a focus of interest as stimulators and regulator of growth and biosynthetic processes of microorganisms producing bioactive compounds. The efficacy of their use can be both beneficial and toxic depending on the composition, size, duration and applied concentration. The purpose of the researches was to study the influence of Cu, Co and ZnO nanoparticles on micromycetes. 20 strains of micromycetes from the National Collection of Non-pathogenic Microorganisms (CNMN) belonging to the genus: Aspergillus (5), Trichoderma (5) and Penicillium( 10) were used as study material. Nanoparticles (NP) of Cu, Co and ZnO with the dimensions: NPCu - 2-3 nm, NPCo-15-20nm, NPZnO - 20-30 nm, were synthesized by the scientific researchers from Southwest State University,Kursk, Russia. Micromycetes were grown on agar and liquid Czapek media with the following NP concentrations (%): Cu and ZnO - 0.001; Co - 0,0001. Cultivation on agar medium was carried out in Petri dishes at 28-30°C for 10 days. During this period the morpho-cultural peculiarities of the studied micromycetes were monitored (size, shape and color of the colonies, sporulation, spores, etc.). The submerged cultivation was carried out in 250 ml Erlenmeyer flasks on a shaker at 180-200 r.p.m. at 28-300° C for 6 days. As a control group, the variant without nanoparticles was investigated. The antifungal activity of the strains, belonging to the Trichoderma genus, was determined by agar blocks method, by the diameter of the growth inhibition zone of the phytopathogen. The following phytopathogens were studied as test-cultures: Aspergillus niger, Alternaria alternata, Botrytis cinerea, Fusarium solani, Fusarium oxysporum. The experiments were performed in 3 times. Statistical data processing was performed using MS Office Excel 2010. The obtained results showed that Cu and ZnO nanoparticles added to liquid culture medium do not significantly change the micromycetes growth, the amount of biomass accumulated in the experimental variants varies within the limits ± 10% in comparison with control variant. The presence of Co NP in the growing medium significantly diminishes the growth of the micromycetes. When grown on agar medium, NP of Cu and ZnO stimulated the growth and modified the mycelium color, and NP of Co inhibited growth in comparison with control group. The study of the antifungal activity of genus Trichoderma cultivated on media supplemented with nanoparticles showed that the tested nanopatricles did not significantly influence the biosynthetic processes of the studied strains. Thus, the metabolites obtained in the variants with addition of nanoparticles showed less antifungal activity on the phytopathogen A. niger compared to the control variant. The sensitivity of Alternaria alternata, B. cinerea and F. solani was at the level of the control. A more pronounced sensitivity was shown by Fusarium oxysporum to the metabolites obtained in variants with Cu and ZnO nanoparticles, the inhibition zones exceeding the control by 4.5-12.8%. According to the obtained results we can conclude that the supplementation of the cultivation medium with Cu and ZnO NP has not significantly influenced the growth, morphocultural properties and antifungal activity of the studied strains; and Co NP have an inhibitory effect on micromycetes growth.