|Ultima descărcare din IBN:|
| SM ISO690:2012|
BIVOL, Cezara; CILOCI (DESEATNIC), Alexandra; TIURINA, Janeta; CLAPCO, Steliana; LABLIUC, Svetlana; DVORNINA, Elena; GUTSUL, Tatiana. Nanomethods for obtaining lipolytic enzyme preparations from fungi. In: Microbial Biotechnology. Ediția 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, pp. 143-144. ISBN 978-9975-3178-8-7.
Ediția 4, 2018
Conferința "Microbial Biotechnology" |
Chișinău, Moldova, 11-12 octombrie 2018
Lipolytic enzymes have important applications in various fields of industry and medicine. Lipases are used to process food, leather, textile fibers, detergents, paper, fine chemicals, pharmaceuticals, cosmetics, biofuels etc. The major lipase producers are fungi from genera Aspergillus, Penicillium, Rhizopus, Candida, Mucor. An innovative concept in the biotechnological production of microbial lipases is the use of nanoparticles as stimulating and regulating factors of biosynthesis. The nanoparticles present a unique tool in manipulation of the biosynthetic activity of microorganisms with proven efficiency on biotechnological objects from different taxonomic groups. The aim of this research was to develop methods for obtaining new lipolytic preparations with the application of nanoparticles as factor of influence. The object of the study was Aspergillus niger CNMN FD 01 fungi strain with biotechnological significance, with high and stable synthesis of exocellular lipase (MD 2362). The culture was stored in the National Collection of Nonpathogenic Microorganisms of the Institute of Microbiology and Biotechnology, Moldova. In order to increase the biosynthetic capacity of A. niger, the influence of TiO2 (of 20nm, 40nm, 1μm) and Fe3O4 (of 10nm, 30-35nm, 70nm) metal oxides were tested. Nanoparticles were included into culture medium in the following concentrations: 1.0; 5.0; 10.0 and 15.0 mg/L. In conclusion, we developed an integrated scheme for obtaining new lipolytic enzyme preparation with a 10x degree of purity, having a lipolytic activity of 75.000 U/g and a specific activity of 2205 U/mg protein. It included the cultivation of Aspergillus niger CNMN FD 01 strain in the presence of 10mg/L TiO2 nano-oxide with dimension of 40 nm, followed by lipase separation through sedimentation with 96% ethanol. Obtained lipolytic preparation can be used in zootechny, food industry, light industry and bioremediation processes.