Bioremediation of polluted environments is based on contaminant biodegradation, that is, metabolic abilities of microorganisms to transform or mineralize organic contaminants into less harmful, nonhazardous substances, which are further integrated into natural biogeochemical cycles. Rhodococcus species are ubiquitous in pristine and contaminated environments, possess remarkable metabolic activities, can persist under harsh environmental conditions, compete successfully in complex bacterial populations, and therefore could be considered as having great potential in bioremediation applications. Upon revealing new catabolic abilities of Rhodococcus species and isolation of environmental strains degrading a wide range of contaminants, these bacteria have been increasingly explored for bioremediation of soils, waters, and air polluted with different recalcitrant and toxic organic chemicals. Using microbial cells, immobilized for the purpose of biosynthesis or biodegradation reactions remains one of the most researched technologies of recent years. Numerous biotechnological processes are advantaged by immobilization techniques and therefore several such techniques and support materials have been proposed. Immobilized cells are frequently used for the biotransformation due to advantages such as the prevention of the elution of impurities from the cells, easy separation of the cells from a reaction mixture, repeated use of the immobilized cells, and enhanced stability of the cells. For cell immobilization are used media types and methods well studied in the last two decades. However, the methods of incorporating microbial cells into different gels such as polyacrylamide gel, alginate, carrageenan, polyvinyl alcohol, are predominant. The immobilization supports are classified as inorganic (montmorillonite, zeolite, diatomite, different clays, anthracite, porous glass, activated charcoal, etc.) and organic as cellulose (DEAE-cellulose), wood sawdust, delignified sawdust etc. Inorganic supports have been selected to immobilize microorganisms because they can survive microbial degradation and are thermostable [Verma, 2010; Bayat, 2015]. The main objective of our research was to provide a simple, non-expensive and efficient carrier for the preparation of immobilized rhodococci cells. The strain Rhodococcus rhodochrous, deposited in the National Collection of Non-pathogenic Microorganisms of the Republic of Moldova as Rhodococcus rhodochrous CNMN-Ac-05, was used as a object of study. The rhodococci cells were immobilized on 1 g of solid support according to Kitova et al., 2004. The amount of immobilized cells was estimated by two different methods: indirect, or spectrophotometrically, by measuring the D540 optical density of the liquid medium before and after immobilization, and direct, by quantifying the number of viable bacterial cells (colony forming units, CFU) inoculated on Tryptic Soy Agar medium before and after immobilization. The inorganic supports, such as bentonite and kieselgur, were selected as excellent supports for adsorptive immobilization of R. rhodochrous cells (69.3% and 97.3% of bacterial immobilization, respectively). Readily available, low-cost organic carriers, such as shells from walnut, hazelnut, pistachio, peanut, husks from pumpkin and sunflower seeds, were tested as supports for immobilization of R. rhodochrous cells. The shell from peanuts as support material demonstrated a good adsorption of bacteria cells – 34.3%, while cells immobilization on the husk from sunflower seeds was very low – 6.2%.