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TSAPKOV, Viktor; COTOVAIA, Aliona; MILENTIEV, A.; BURDUNIUC, Olga; BALAN, Greta; GULEA, Aurelian; MITKEVICH, N.. Antimicrobial and antifungal effect of some biometal coordination compounds with 2-[(3-methyl-5-oxo-1-phenyl-4,5-dihydro-1H-pyrazol-4-yl)-(phenyl) methylidene] hydrazine carboximidamide. In: Microbial Biotechnology. Ediția 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, pp. 55-56. ISBN 978-9975-3178-8-7.
Ediția 4, 2018
Conferința "Microbial Biotechnology" |
Chișinău, Moldova, 11-12 octombrie 2018
The aim of this work is the determination of antimicrobial and antifungal activities of manganese, iron, cobalt, nickel, and copper coordination compounds with 2-[(3-methyl- 5-oxo-1-phenyl-4,5-dihydro-1H-pyrazol-4-yl)(phenyl)methylidene]hydrazinecarboximidamide (HL). Chlorides, nitrates and acetates of stated above metals form coordination with the following composition: M(L)2X (M = Fe3+, Co3+, X = Cl-, NO3 -), ML2 (M = Mn, Ni) and Cu(L)X(X = Cl-, NO3 -, CH3COO-). The antimicrobial activity of these compounds was studied in vitro using the method of two-fold serial dilutions in liquid nutrient medium (meat infusion broth, pH = 7.0). The substances were dissolved in DMSO in such amounts that the solutions of concentration 10 mg / mL were obtained. The next dilutions were made using meat infusion broth. The antimicrobial activity was tested on standard strains of gram-positive (Staphylococcus aureus ATCC 25923), gram-negative (Escherihia coli ATCC 25922) bacteria and Candida albicans. 18-Hours agar culture of these microorganisms was used for bacterial inoculation. Inoculation dose of 250- 500 ths. microorganisms per 1mL was determined by optical turbidity standard. Test tubes were agitated and placed in thermostat at 370C for 24h. The test tube with nutrient medium and microorganisms but without the studied substance was used as control sample. If there were no signs of visible growth of microorganisms in the liquid nutrient medium then the tested substance was defined as bacteriostatic at this concentration. For detecting bactericidal activity of tested substance microorganisms were reinoculated on a solid nutrient medium (meat infusion agar) and incubated. The absence of visible growth of microorganisns indicates the bactericidal activity of tested substances at this concentration. It was determined that the synthesized coordination compounds manifest selective bacteriostatic and bactericide activity towards both gram-positive and gram-negative microorganisms in the range of concentrations 0.016-1.0 mg / mL. The experiment showed that the nature of the central atom and acid residue have the main influence on the minimal inhibitory (MIC) and bactericidal (MBC) concentrations of the studied coordination compounds. For the homotypic coordination compounds MICs and MBCs change in the following way: Сo3+ ³ Ni2+ > Mn2+ > Cu2+ > Fe3+ and Cl- > NO3 - > Br- > CH3COO.