Previously coordination compounds of bio-metals with the 4methoxyphenylthiosemicarbazones of the substituted pyridine-2-carboxaldehyde. It has been established that te initial ligands and their coordination compounds possess selective anticancer activity have been synthesized. Further on it has been of great interest to synthesize new compounds of some 3d metals with such type of ligands, to study their composition and structure, and also their physico-chemical and biological properties. As a result there have been synthesized 24 new coordination compounds of Co(III), Ni(II), Cu(II) and Zn(II) with the 2-, 3- and 4-methoxyphenylthiosemicarbazones of the pyridine-2-carboxaldehydes. For these compounds it has been established the composition, based on the elemental and physico-chemical study: M(L)X and [Co(L)2]X, where M = Ni(II), Cu(II), Zn(II); X = Cl-, NO3 -. Based on the magnetochemical research of the synthesized coordination compounds it has been established that in the synthesis process Co(II) oxidizes to Co(III). The studied thiosemicarbazones act as ligands of strong field, the nickel complexes possess plainsquared structure and the complex compounds of copper possess a monomeric structure. The comparative analysis of the IR spectra of the complex compounds with the ones of the thiosemicarbazones show that the studied thiosemicarbazones within the synthesized complex compounds act like tridentate ligands, bounding with the central ion through pyridine and azomethine nitrogen atoms and sulphur atoms, forming two metalcycles containing five atoms each. Synthesized coordination compounds show selective antimicrobial activity towards a series of standard strains Staphylococcus aureus (ATCC 25923), Enterococcus faecalis, Escherihia coli (ATCC 25922), Salmonella abony (GISK 03/03), and Candida albicans in the range of concentration 0.03-2.0 mg/mL. The experimental data obtained on studying the antifungal properties of copper chloride (II) with 4-(2-methoxyphenyl)- 4-(3-methoxyphenyl)and 4-(4-methoxyphenyl)-2-benzoylpyridine thiosemicarbazones of general formula: Cu N N S N NH R1 R2 R3 Cl I-III I : R1 = OCH3, R2 = R3 = H; II : R1 = R3 = H, R2 = OCH3; III : R1 = R2 = H, R3 = OCH3 demonstrated that these exhibit activity against laboratory strains of Candida albicans concentrations within 0.59 to 37.5 mg/ml. Identified properties of interest for medical and veterinary practice in terms of extending the arsenal of antifungal remedies.