Microbial proteases are an important group of useful enzymes with multiple applications in various industrial and biotechnological sectors such as additives in detergents, leather and food industries, organic synthesis and pharmaceuticals, environmental bioremediations, biotransformation and molecular biology1. Previous studies to increase biosynthesis of fungi exocellular hydrolases (lipases, cellulases, amylases, pectinases) have revealed stimulatory effect of some metal complexes on growth and enzymogenesis of mycelial fungi, reducing the technological cycle and directing differentially obtaining of enzyme products. The aim of the present research was to evaluate the effect of metal complex [Cu(DSamH2)3]SO4·5H2O on proteolytic activity of Fusarium gibbosum CNMN FD 12 fungal strain – producer of acid and neutral proteases. The coordinative compounds [Cu(DSamH2)3]SO4·5H2O, were synthesized on the basis of a new α-dioxime ligand (DSamH2 disulfanilamideglyoxime) and the structure has been determined by elemental analysis, IR, and X-ray crystallography. In this oxime the sulfanilamide is condensed via NH2 group closer to aromatic ring. In the crystal a supramolecular network are formed due to numerous H-bonds between donor-acceptor atoms of cations, anions and crystalization water molecules2. The compound was added to nutrient medium in concentration of 1, 5, 10, 15 mg/L. Activity of neutral (pH-7.4) and acid (pH-3.6) proteases was determined according to the Willstatter method, using 5% gelatine as substrate. The influence of coordination compounds was evaluated in dynamics, during the 4-6 days of cultivation. The peak of acid and neutral protease activity was determined on the 5th day of cultivation, similar to the control sample. On the 6th day of cultivation fungal proteolytic activity decreased significantly. The optimal concentration for the both types of activity was the minimal tested concentration – 1.0 mg/L, which ensure increasing of activity by 71.7% and 96.0%, for acid and neutral proteases, respectively. The higher concentrations determined the decrease of enzymatic activity, more essential in the case of acid proteases, but not less than in the control. Thus, acid proteases activity of variants cultivated in the presence of 15 mg/L coordinative compound was equivalent to control. Activity of neutral proteases decreases up to 5,712 U/mL, being by 48.0% higher than control. The metal complex [Cu(DSamH2)3]SO4·5H2O intensified the proteolytic activity of Fusarium gibbosum CNMN FD 12 strain and can be recommended as regulators of fungal synthesis.