Biopolymers like deoxyribonucleic acid and collagen show very interesting properties as possible matrices for active molecules, being able to replace synthetic polymers [1]. These biopolymers are extracted from waste of food processing industry and their sources are practically unlimited. Their degradation time is much faster than that of synthetic polymers in similar conditions. On the other hand, during the last time, the scientists turn their attention to natural photosensitive materials, which can be extracted from flowers or fruits, like anthocyanines [2] showing some photosensitive properties for biophotonic applications. In this paper we present and discuss the results on synthesis and characterisation of some all-bio-nanostructured materials by doping DNA with bio-active molecules present in green tea and sea buckthorn extracts. In this context, the deoxyribonucleic acid as such or functionalized with cetyltrimethylammonium chloride (CTMA) surfactant has been doped with green tea or sea buckthorn extracts and processed in good optical quality thin films by spin coating. The optical properties were characterized by UV–VIS and fluorescence spectroscopy. The nonlinear optical properties of functionalized thin films were studied by optical third harmonic generation measurements at 1064.2 nm fundamental wavelength. Both deoxyribonucleic acid and green tea extract are transparent at fundamental wavelength. The complexes exhibit photoluminescence when excited in the UV absorption band. The photoluminescence intensity is significantly larger in DNA-GTE complexes than in GTE alone. The DNA-CTMA-SBE complex exhibits large absorption band in Visible domain, which is due to the SBE chromophores as DNA-CTMA is transparent in visible domain. The results of spectroscopic studies and third harmonic generation measurements indicate the new obtained deoxyribonucleic acid – green tea complex as promising material for blue biological light emitting diodes (BIOLED’s) and for blue lasers (BIOLASERS) fabrication.