The dry hydro-ethanolic extract obtained from leaves of Amaranthus retroflexus L. (Amaranthaceae) with an yield of 22.53% was assessed with respect to its acute toxicity in NMRI mice, cytotoxicity on the crustacean Daphnia magna and phytotoxicity on Triticum aestivum L. The lack of lethality and of any change in the indicators followed in mice (2000 mg/kg b.w. po) was demonstrated, the extract being classified into the 5^th category of the Global Harmonized System (GHS). The Daphnia magna test results indicated a low toxicity (LC50 of 1053.0 µg/mL), in agreement with the results above, and the cytotoxicity and genotoxicity on plant cell, investigated by the Triticum bioassay manifest at high concentrations only (1.00% - 0.100%), for which changes in the mitotic film are also observed: mitoinhibition, anaphases with retarded chromosomes, metaphases with “sticky” chromosomes.