The effect of some biological control agents against combined attacks of the plant pathogen Verticillium dahliae and the root-knot nematode Meloidogyne incognita on eggplant was verified in an open field trial. A soil at Valenzano (Bari province, Apulia region, Italy), naturally infested by the above mentioned pests was deeply ploughed, rotavated and subdivided in 4 m x 4 m plots, spaced 1 m each other and distributed in a randomized block design with three replications for each treatment. Treatments were: a) Clonostachys rosea with fungicidal activity (F) (applied at transplant and 15 days later at 2L/ha) + Paecilomyces lilacinus isolate 251 with nematicidal activity (N) [applied in pre (1 week) and post-transplant (40 days later) at 4 Kg/ha] + Aphanocladium album isolate MX-95 (N activity) (applied 1 week before transplant and 15 days later at 0,4 L/plant as conidial suspension at 1,2 x 107 CFU/mL); b) Clonostachys rosea (applied as before indicated) + Paecilomyces lilacinus isolate 251 (as before indicated) + chestnut tannins (applied at transplant at 3,2 g/plant); c) A. album MX-95 (applied as above mentioned); d) chestnut tannins (applied as in b treatment); e) A. album MX-95 + chestnut tannins (applied as in a and b treatments) and f) Azoxystrobyn (F activity) at 20 L/ha + Fenamiphos EC (N) at 62.5 L/ha, both applied at transplant. Untreated plots were used as control. The biological control agents, chestnut tannins and chemicals were applied by injector in localized form to the base of each plant in correspondence of water emitters of PVC drip irrigation lines. In each plot there were three rows of plants with 6 plants/row. During the growing season the crop received the cultural practices that are common for the area. Fruits were harvested (nine times) during crop cycle and yield recorded. At the end of the experimental trial, plants were uprooted to estimate root gall index caused by the nematode attack according to a 0-5 scale (0= health root system and 5 = root system completely deformed by large and numerous galls). Severity of Verticillium wilt was assessed according to a 0-5 scale (0 = health plant; 1 = yellowing of the basal leaves; 2 = widespread yellowing; 3 = wilting; 4 = widespread wilting and 5 = death plant). Stem of each plant was transversely cut 3 cm above the soil level and the severity of vascular discoloration (VD, % area affect) was evaluated according to a 0-4 scale (0= no vascular discoloration; 1 = 1-10% VD; 2 = 11-50 % VD; 3 = 51-75 %VD and 4 = 76-100% VD). Nematodes from each plot were extracted from soil samples processing 0,5 L soil sub-sample with the Coolen’s method. Data were statistically analyzed and means compared by Duncan’s Multiple Range Test. All treatments had a positive influence on yield and were effective for a significant reduction of severity of symptoms of Verticillium wilt, vascular discoloration and M. incognita attacks in comparison to the untreated control (Table 1). On the base of our results it is reasonably possible consider the use of biological control agents in Verticillium wilt and plant parasitic nematode sustainable control, although further investigation are suggested in different areas, crops, periods and types of soil. Aknowledgement: The research was undertaken within the framework of the bilateral project nr. 15.820.18.05.07/It between the National Research Council (CNR-Italy) and the Academy of Science of R. Moldova nr. 15.817.02.12F.