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SM ISO690:2012 НИКОЛАЕВ, Аркадий, НИКОЛАЕВА, Светлана. Опыт создания сухой препаративной формы биопрепарата на основе Bacillus subtilis. In: Actual problems of zoology and parasitology: achievements and prospects, 13 octombrie 2017, Chișinău. Chișinău, Republica Moldova: Institutul de Zoologie, 2017, pp. 265-269. ISBN 978-9975-66-590-2. DOI: https://doi.org/10.53937/9789975665902.58 |
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Actual problems of zoology and parasitology: achievements and prospects 2017 | ||||||
Simpozionul "Actual problems of zoology and parasitology: achievements and prospects" Chișinău, Moldova, 13 octombrie 2017 | ||||||
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DOI:https://doi.org/10.53937/9789975665902.58 | ||||||
Pag. 265-269 | ||||||
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The aim of the studies was to create a dry preparative form of a biopreparation based on Bacillus subtilis spores and the determination of an acceptable shelf life without significant loss of the spores viability. The technology for obtaining a dry formulation includes the following steps: cultivation of the bacteria B. subtilis in a liquid nutrient medium to the stage for the maximum sporulation; separation of spore biomass from the nutrient medium; adsorption of spores on a mixture of starch and powdered sugar and , if necessary, the subsequent addition of powdered sugar till creation a pulverulent stage. A mixture of sugar and starch with biomass ratio of bacteria spores and starch not more than 1:1 and biomass of bacteria spores and sugar not more than1:50 was used as a carrier. The storing of the drug in a plastic vial with a screw cap at room condition in the laboratory during 5 month has not changed the titre of spores in the preparation. The spore titre decreased from 8,6*109 spores/1 g to 5,7*109 spores/1 g when preparation was stored in the same condition for three years. The dry powder composition not only contributes to the preservation of spores in a viable state, but also provides a source for spores feeding when spraying plants with drug. |
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Cuvinte-cheie dry preparation form, spores viability, , Shelf life, Bacillus subtilis |
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