Considering approaches for development of regional and national optical networking infrastructure for providing access to services and technologies for the research and educational communities offered by Pan-European and regional e-Infrastructures. Development of regional connectivity and transferring of the national research and educational optical network in Moldova to use new technologies supported by European Eastern Partnership Programme. Over the several past years demands of research and education institutions for access to high-speed networking infrastructures, to large-scale computing and other e-Infrastructures’ services are rapidly increasing. Modern e-Infrastructures integrating various ICT based resources, services and considered as a key enabler for scientific and social development. In the paper describing realization of solutions that are necessary for integrating various national e-Infrastructures and providing convenient, high speed and reliable access to various services that are deploying in Europe to support research and educational activities. Argued importance of National Research and Educational networks (NREN) as a key instruments for access to e-Infrastructures’ resources and one of the most important supporters of e-Infrastructure development.