The seed morphology of 38 samples representing O. cumana populations from Republic of Moldova were examined to explore patterns of variation among the accessions. Features of seeds were assessed employing light microscopy and analyzed statistically. The morphological differences in length/ width ratio and seed shape in local broomrape populations have been established. The size of seeds ranged from 0.36 ~ 0.42 mm in length, and 0.14~0.18 mm in width. Seeds were generally small, ovoid to elongated in shape. Using one-way test (ANOVA), cluster and principal coordinate analysis, two groups were established by seed characteristics.