Studies regarding micropropagation of hybrid berry cultivars Tayberry Medana and Tayberry Buckingham
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581.1:634.71:631.589 (1)
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MÎRZA, Alexandru. Studies regarding micropropagation of hybrid berry cultivars Tayberry Medana and Tayberry Buckingham. In: Revista Botanică, 2016, nr. 1(12), pp. 12-16. ISSN 1857-2367.
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Revista Botanică
Numărul 1(12) / 2016 / ISSN 1857-2367 /ISSNe 2587-3814

Studies regarding micropropagation of hybrid berry cultivars Tayberry Medana and Tayberry Buckingham
CZU: 581.1:634.71:631.589

Pag. 12-16

Mîrza Alexandru
 
Botanical Garden (Institute) of the Academy of Sciences of Moldova
 
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Disponibil în IBN: 2 decembrie 2016


Rezumat

Abstract. Objective: Here, we established the protocol for plant regeneration of Rubus loganobaccus L.H. Bailey via in vitro micro propagation. Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented with different concentrations of plant growth regulators, 6-Benzylaminopurine (BAP). Results: For both cultivars, Tayberry Medana and Tayberry Buckingham, good results were obtained for 0.3 and 0.4 mg/l BAP concentration. The micro-shoots produced normal roots within two weeks of culture on the 50% MS medium with no supplement of plant growth regulators. Plantlets were transferred to celled trays where they grew well. Afterwards the plants were planted in the field where they continued the development. Conclusion: The micro propagation protocol reported here was characterized by a rapid proliferation of shoots, easy rooting of the micro-shoots and the plantlets were easily acclimatized to the external environment and undergoing normal physiological development.

Cuvinte-cheie
Cytokinin, 6-Benzylaminopurine (BAP), Micropropagation, MS medium, Rubus loganobaccus, rooting,

microshoots