Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
1054 7 |
Ultima descărcare din IBN: 2024-01-26 14:57 |
Căutarea după subiecte similare conform CZU |
575.8 (19) |
Genetică generală. Citogenetică generală (427) |
SM ISO690:2012 CEPOI, Liliana, GOLAN, Jacob, GRYGANSKYI, I. Phylogenetical approach for the search of
valuable metabolic products in cyanobacteria. In: Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii, 2015, nr. 2(326), pp. 167-172. ISSN 1857-064X. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii | ||||||
Numărul 2(326) / 2015 / ISSN 1857-064X | ||||||
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CZU: 575.8 | ||||||
Pag. 167-172 | ||||||
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Rezumat | ||||||
Based on CrtP gene analysis we showed how phylogenetic approach could be used for detection of potentially efficient carotenoids producers, identification of their unknown isolates and primer design for the screening of useful genes. ML analysis of the gene CrtP1in 15 taxa of cyanobacteria has shown that two cyanobacteria groups – Anabaena and Nostoc might be artificial and polyphyletic. More isolates and genes should be included in such analysis to detect possible horizontal gene transfer events in evolution of branching filamentous cyanobacteria. Phylogenetic analysis can be helpful in identification of unknown isolates. So, on our tree CrtP gene of unknown bacterium (clone 66415, acc. # KP445989) is nested together with the gene of Nostoc punctiforme and possibly represents the same or closely related species. For CrtP gene we have determined the fragment with total length 1350 nucleotide basepairs (450 amino acids) with no intones and pretty uniform structure. Both starting and ending part of this fragment with length of 39 nucleotide basepairs are well conserved (same aminoacids with no changes or minor differences in 3rd codon positions) are very suitable for primer design. 20 references, 3 figure, 1 table. |
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Cuvinte-cheie 16S rRNA gene, Cyanobacteria, CrtP gene, phylogenetical approach. |
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