Impact of sucrose concentration on in vitro culture long-term storage of rare species
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2024-01-02 14:14
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581.1(478) (13)
Fiziologia plantelor (522)
SM ISO690:2012
SEDCENCO, Maria. Impact of sucrose concentration on in vitro culture long-term storage of rare species. In: Revista Botanică, 2015, nr. 1(10), pp. 46-50. ISSN 1857-2367.
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Revista Botanică
Numărul 1(10) / 2015 / ISSN 1857-2367 /ISSNe 2587-3814

Impact of sucrose concentration on in vitro culture long-term storage of rare species
CZU: 581.1(478)

Pag. 46-50

Sedcenco Maria
 
Botanical Garden (Institute) of the Academy of Sciences of Moldova
 
Disponibil în IBN: 13 mai 2015


Rezumat

This article describes the peculiarities of long-term in vitro preservation of rare spontaneous plants from Moldova. In our experiment, we have used a low-cost substance, i.e. sucrose, as an agent that inhibits growth of plantlets in tubes and increases their viability after long-term storage. The plantlets were maintained in an environment without any phytohormones applied while various concentrations of sucrose were used. After 12 months of cultivation, the amount of regenerated plants which survived was assessed, and their visual analysis was performed. To maintain Lilium martagon L. as a species in vitro for a long term, MS-100% environment supplemented with sucrose concentration of 100 g/l was applied. To maintain Fritillaria montana and Bellevalia sarmatica as a species, according to the viability estimates, the most optimal environment is MS- 100% with sucrose in an amount of 90 g/l. Hence, higher sucrose concentrations in nutrient environments can be used successfully for in vitro long-term maintenance of rare species.