Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
637 13 |
Ultima descărcare din IBN: 2024-01-02 14:14 |
Căutarea după subiecte similare conform CZU |
581.1(478) (13) |
Fiziologia plantelor (522) |
SM ISO690:2012 SEDCENCO, Maria. Impact of sucrose concentration on in vitro culture long-term storage of rare species. In: Revista Botanică, 2015, nr. 1(10), pp. 46-50. ISSN 1857-2367. |
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Revista Botanică | |||||
Numărul 1(10) / 2015 / ISSN 1857-2367 /ISSNe 2587-3814 | |||||
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CZU: 581.1(478) | |||||
Pag. 46-50 | |||||
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Rezumat | |||||
This article describes the peculiarities of long-term in vitro preservation of rare spontaneous plants from Moldova. In our experiment, we have used a low-cost substance, i.e. sucrose, as an agent that inhibits growth of plantlets in tubes and increases their viability after long-term storage. The plantlets were maintained in an environment without any phytohormones applied while various
concentrations of sucrose were used. After 12 months of cultivation, the amount of regenerated plants which survived was assessed, and their visual analysis was performed. To maintain Lilium martagon L.
as a species in vitro for a long term, MS-100% environment supplemented with sucrose concentration of 100 g/l was applied. To maintain Fritillaria montana and Bellevalia sarmatica as a species, according to the viability estimates, the most optimal environment is MS- 100% with sucrose in an amount of 90 g/l. Hence, higher sucrose concentrations in nutrient environments can be used successfully for in vitro long-term maintenance of rare species. |
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