In the Dniester Basin have been concentrated the most water resources, urban centers, industrial and agricultural zones of the Republic of Moldova. The absolute majority of the surface waters captured from Dniester river basin are destined for a reduced number of localities. The rest of localities, situated in the perimeter of the basin, capture the water, almost exclusively, from the underground sources, the Dniester river and its tributaries have the only destination of natural receptors of the waste waters. The main topics presented in this paper are: 1) the dynamics of volume of captured and used waters of the Dniester basin and its sections; 2) fields of water use, 3) spatial and branch profile of used waters: 4) existing problems in evaluation and monitoring of used water; 5) irrevocable loss of water; 6) water supply services.