Evaluation of the genetic diversity of Origanum genus’ species
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MARTEA, Rodica; MUTU (CALMÎŞ), Ana; CLAPCO, Steliana; BUDEANU, Oleg; GILLE, Elvira. Evaluation of the genetic diversity of Origanum genus’ species . In: Revista Botanică. 2014, nr. 2(9), pp. 125-130. ISSN 1857-095X.
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Revista Botanică
Numărul 2(9) / 2014 / ISSN 1857-095X

Evaluation of the genetic diversity of Origanum genus’ species

Pag. 125-130

Martea Rodica1, Mutu (Calmîş) Ana1, Clapco Steliana1, Budeanu Oleg1, Gille Elvira2
 
1 University of the Academy of Sciences of Moldova,
2 Center of Biological Sciences „Stejarul”, Piatra Neamţ
 
Disponibil în IBN: 12 ianuarie 2015


Rezumat

Origanum belonging to the Lamiaceae family is one of the most wide-spread genera in the spontaneous flora of many landscapes. It has a precious natural resource, as it provides raw material for pharmaceutical industry, modern and traditional forms of medicine and generates employment and income in addition to conservation of biodiversity. The RAPD - PCR analysis of genetic polymorphism in two species of the Origanum genus – O. vulgare L. and O. laevigatum Boiss. collected from a region of spontaneous flora in Moldova (Orheiul-Vechi) indicated a high heterogeneity of the results by tested primers. Based on the electrophoretic spectrum, 82 amplification products were identified with an average of 11,71 bands per primer. The comparative analysis of molecular polymorphism across the named species revealed that the RAPD-PCR generated 45 bands for O. vulgare L. and 37 for O. laevigatum Boiss. 33 of them are polymorphic and 46 – monomorphic fragments. The obtained results allowed concluding that the most informative primers are: for O. vulgare L. – OPG5 and for O. laevigatum Boiss. - OPA19 showing a maximum number of fragments (7, respectively 10). The most specific bands were revealed in the case of OPA19 O. vulgare L.- 7 and O. laevigatum Boiss. 6 decamers.