Background: Opportunistic infections of mycotic etiology are included in the group of new infectious diseases, and their proportion is higher in the framework of infectious diseases, especially, in the context of the misuse of antibiotics synthesis. Material and methods: 72 samples, collected from women with various gynecological diseases have been examined. In 41 cases the fungi with Candida spp. have been detected. Results: The nutrient, which allows a rapid detection of fungus Candida spp. has been developed. For the first time has been developed a nutrient medium of a new culture in the form of a micro film for isolation, multiplication and a rapid identification of yeast form fungi of Candida species, which allows to detect the microbes beginning from the time of 4-5 hours to 9-24 hours depending on their initial concentration in 1 ml; individual cells need more than 9-24 hours and the concentrations of 103-104 c.m./ml are detected within 4-5 hours of the incubation period at the temperature of 370C and higher. The medium MDC-Cand has a selectivity, mainly for yeast form fungi of Candida species. Conclusion: This nutrient medium is sensitive, economical and simple for the usage in microbiological laboratories of various levels. The period of storage of the medium is 2 years.