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SM ISO690:2012 RÎMIŞ, Constantin, SOFRONIE, Olga, SCUTARU, Andrei. Etiologia infecţiilor de plagă şi rezistenţa la antibiotice. In: Curierul Medical, 2013, nr. 5(56), pp. 148-152. ISSN 1875-0666. |
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Curierul Medical | |||||
Numărul 5(56) / 2013 / ISSN 1875-0666 | |||||
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Pag. 148-152 | |||||
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This paper comprises the analysis of laboratory results on the etiology of wound infections and the antibiotic sensitivity of the microorganisms detected during the period of 2010-2012. Among all the detected microorganisms, gram-positive bacteria have been 56.3% and gram-negative bacteria – 43.0%. The bacteria mostly identified in all the isolated species have been S. aureus – 35.1%. An increased multidrug resistance of the detected microorganisms during the studied period of three years has been determined, which is an indicator of an improper administration of treatment, that is, the research of the strain sensitivity to antibiotics or the proper observance of the terms of antibiotics use has not been realized. This study is based on a retrospective analysis of the reports on this issue and antibiograms for the last 3 years (2010-2012). A total of 993 samples (allocation of ulcers) with infectious agents with clinical signs, received from the branches of the surgical profile of medical institutions of Chisinau municipality, have been included into the study. The isolation of the pathogens has been conducted by selective and enriched methods, as well as by a chromogenic method that allows a direct and rapid identification. The Identification of the microorganisms isolated from ulcers has been conducted on the basis of morphological data and through the biochemical tests Microbact (Oxoid). Antibiotic susceptibility testing has been performed with the help of the classical qualitative methods: diffusion metrical technique on the subculture, adjusted to the standard McFarland 0.5, Kirby-Bauer method with the
use of Mueller Hinton’s medium and micro pills of antibiotics, the manufacturer Himedia (India). |
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Cuvinte-cheie wound, drug resistance, Antibiotics, microorganisms. |
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