Contribution in studying chemical-toxicology of aminophylline in biotransformation process The main idea of this work was to develop physical-chemical methods by investigation theophylline Isolation tests from biological liquids were affected in PH diversions. Results confirmed by thin layer of chromatography pplication.Application of mobile phases of methanol-sol.ammonium25% (100-1.5) with Rf 0.75,and UV spectrophometry with maximum absorbtionby wave length 275 nm, IR spectroscopy (suspension in oil of Vaseline ) with band absorbtion 1670,1717,1567,715,980 cm (KBr) Identification of theophylline was determined by HPLC method.This method implicates coloumn C 18 Lichrospher 100-RP-18C with precoloun Lichrocort-124-4 in mobile phase trietilamine 146 μl , Phosphoric acid 750 μl, Water till 530 ml,PH-3,3 with Na0H solution 10% and apply Acetonitril till 750 ml. Current rate is 0.6 ml/min

Scopul lucrării a fost de a dezvolta metodele fizico-chimice de cercetare asupra teofilinei. Probele de izolare din lichidele biologice au fost efectuate la diverse pH-uri. Rezultatele s-au confirmat prin aplicarea cromatografiei pe strat subţire, aplicănd fazele mobile metanol – sol. amoniac 25 % (100 : 1,5) cu valoarea Rf 0,75, şi spectrofotometria UV cu maxim de absorbţie la lungimea de undă 275 nm, spectoscopia IR (suspenzie în ulei de vaselină) cu benzile de absorbţie la 1670, 1717, 1567, 745, 980 cm-1 (KBr). Identificarea teofilinei s-a determinat şi prin metoda HPLC aplicănd coloana C18 Lichospher 100-RP-18 C cu precoloană Lichrocort 124-4 în faza mobilă trietilamină 146 μl,, acid fosforic 750 μl, apă pînă la 530ml, se ajustează pH 3,3 cu soluţie NaOH 10% şi se aduce cu acetonitril la 750ml. Rata curentului 0,6 ml/min.