Caracterizarea fazeolinei modificate in vivo
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MORARI, Diana, STEPURINA, Tatiana, ROTARI, Vitalie. Caracterizarea fazeolinei modificate in vivo. In: Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii), 2011, nr. 6(46), pp. 51-57. ISSN 1814-3237.
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Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii)
Numărul 6(46) / 2011 / ISSN 1814-3237 /ISSNe 1857-498X

Caracterizarea fazeolinei modificate in vivo

Pag. 51-57

Morari Diana, Stepurina Tatiana, Rotari Vitalie
 
Universitatea de Stat din Moldova
 
Disponibil în IBN: 17 decembrie 2013


Rezumat

Phaseolin, the 7S seed storage protein from common beans (Phaseolus vulgaris L.), partially modified in vivo during seed germination and seedling growth was isolated from cotyledons of 6 day germinated seedlings (phaseolininvivo). SDS-PAGE showed that the pattern of fragments of phaseolin-invivo is similar to that of the phaseolin degraded by legumain - phaseolin-LLP. Native electrophoresis showed that phaseolin-invivo consists of four molecular forms. 2-D electrophoresis showed that the molecular form of phaseolin-invivo that is similar to phaseolin-LLP retains in its quaternary structure some of the fragments detected by SDS-PAGE. Phaseolin-invivo retains the property to reversibly associate/dissociate with the changes in pH. At pH 4.6 it is present in its dodecameric form while at pH 8.0 in its protomeric form. The implications of the finding that phaseolin-invivo retains the same property of native phaseolin to associate into dodecamers at acidic pHs are discussed.