Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
838 4 |
Ultima descărcare din IBN: 2018-05-08 14:05 |
SM ISO690:2012 MORARI, Diana, STEPURINA, Tatiana, ROTARI, Vitalie. Caracterizarea fazeolinei modificate in vivo. In: Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii), 2011, nr. 6(46), pp. 51-57. ISSN 1814-3237. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii) | |||||
Numărul 6(46) / 2011 / ISSN 1814-3237 /ISSNe 1857-498X | |||||
|
|||||
Pag. 51-57 | |||||
|
|||||
Descarcă PDF | |||||
Rezumat | |||||
Phaseolin, the 7S seed storage protein from common beans (Phaseolus vulgaris L.), partially modified in vivo
during seed germination and seedling growth was isolated from cotyledons of 6 day germinated seedlings (phaseolininvivo).
SDS-PAGE showed that the pattern of fragments of phaseolin-invivo is similar to that of the phaseolin degraded
by legumain - phaseolin-LLP. Native electrophoresis showed that phaseolin-invivo consists of four molecular
forms. 2-D electrophoresis showed that the molecular form of phaseolin-invivo that is similar to phaseolin-LLP retains
in its quaternary structure some of the fragments detected by SDS-PAGE. Phaseolin-invivo retains the property to
reversibly associate/dissociate with the changes in pH. At pH 4.6 it is present in its dodecameric form while at pH 8.0 in
its protomeric form. The implications of the finding that phaseolin-invivo retains the same property of native phaseolin
to associate into dodecamers at acidic pHs are discussed. |
|||||
|