The basic objective of the research consisted in the elucidation of the problems related to the innovative activities performed by the agro-food enterprises, as innovation ecosystem's actors. In order to achieve the proposed objective, an analysis of the economicfinancial situation and of the innovative activities of the agri-food sector of the Republic of Moldova was carried out. As a result, the problems related to the performance of the agro-food sector enterprises and the deficiencies in exercising the innovation function by them were identified and, consequently, recommendations to remedy the identified problems were formulated. The research was carried out within the project "Predictive approaches to increasing the quality of skills in agricultural higher education based on the partnership with the business environment" , No. 20.80009.0807.41, contracting authority - National Agency for Research and Development.