It has been established that bacteriological pollution with concentrations of thermotolerant coliform bacteria and total coliform bacteria was recorded in all tributaries, however, the level of pollution varies significantly, which indicates a significant degree of anthropogenic load along the rivers and different volumes of pollutants. It has been proved that the highest degree of pollution on the right side of the Dniester is for the Byk tributary, and on the left side of the river, it was found for the Svetlyi stream, but its degree of bacteriological pollution is lower than in the Byk tributary. According to the estimated bacteriological indicators, the degree of pollution of the tributaries was characterized and classified from “moderate” to “extremely high” pollution level, which indicates a possible discharge of domestic wastewater into tributaries from households located along the coast that do not have a centralized network of domestic sewerage.