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<creators>
<creator>
<creatorName>Stratan, V.G.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Popa, C.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Sîtnic, V.I.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Ţuţuianu, V.G.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Cebotari, D.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
<creator>
<creatorName>Cojocari, C.</creatorName>
<affiliation>IMSP Institutul Oncologic, Moldova, Republica</affiliation>
</creator>
</creators>
<titles>
<title xml:lang='en,ro'>Periodontal pathogenic analysis in the oral microbioma at patients with head and neck scuamous cell carcinoma</title>
</titles>
<publisher>Instrumentul Bibliometric National</publisher>
<publicationYear>2022</publicationYear>
<relatedIdentifier relatedIdentifierType='ISSN' relationType='IsPartOf'>2345-1467</relatedIdentifier>
<subjects>
<subject>oral microbiome</subject>
<subject>periodontal pathogenic</subject>
<subject>squamous
cell carcinoma of the head and neck</subject>
<subject>microbiom oral</subject>
<subject>agenților patogeni parodontali</subject>
<subject>carcinom cu celule scuamoase cap-gât</subject>
<subject schemeURI='http://udcdata.info/' subjectScheme='UDC'>616.31-022-078:617.51/53-006.61-092</subject>
</subjects>
<dates>
<date dateType='Issued'>2022-10-21</date>
</dates>
<resourceType resourceTypeGeneral='Text'>Journal article</resourceType>
<descriptions>
<description xml:lang='en' descriptionType='Abstract'><p>Background. Research on the relationship between the human microbiome and malignant tumors has received particular interest in recent years. Regarding the oral microbiota, there are different types of bacteria that are associated with oncogenesis of head and neck squamous cell carcinoma (HNSCC). Objective of the study. The purpose of our study was to determine the concentration of periodontal pathogens in the oral microbiome in patients with HNSCC. Material and methods. 26 saliva and oral buffer samples were analyzed from patients with HNSCC by quantitative PCR method. A comprehensive quantitative research kit &bdquo;Dentoscreen&rdquo; (Litech) was used, which allows the identification of the DNA of 7 periodontal pathogens: Porphyromonas endodontalis, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Treponema denticola, Fusobacterium nucella. Results. Show that F. nucleatum exceeds the norm in 20 samples and T. forsythia in 18. F. nucleatum is found in high concentrations in most smokers and only in one patient in the &ldquo;Non-smokers&rdquo; category. Samples with abnormal concentrations of A. actinomycetemcomitans show high concentrations for most species. In addition, the integration with clinical data indicates the pathological presence of P. endodontalis at all patients with laryngeal carcinoma and approx. in 30% of patients with lip and oropharynx carcinoma. Conclusion. Of all the 7 species studied, F. nucleatum and T. forsythia have pathological concentrations in most samples.</p></description>
<description xml:lang='ro' descriptionType='Abstract'><p>Introducere. Cercetările privind relația dintre microbiomul uman și tumorile maligne au &icirc;nregistrat un interes deosebit &icirc;n ultimii ani. &Icirc;n ceea ce privește microbiota orală, există diferite tipuri de bacterii care sunt asociate cu oncogeneza carcinomului cu celule scuamoase de cap și g&acirc;t (HNSCC). Scopul lucrării. Determinarea concentrației agenților patogeni parodontali &icirc;n microbiomul oral la pacienții cu HNSCC. Material și Metode. Au fost analizate 26 de probe de salivă și tampon bucal de la pacienți cu HCSCC prin metoda PCR cantitativ. S-a utilizat un kit cuprinzător pentru cercetare cantitativă &bdquo;Dentoscreen&rdquo; (Litech), care permite identificarea ADN-ului a 7 agenți patogeni parodontali: Porphyromonas endodontalis, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia și Tannerella forsythia. Rezultate. Arată că F. nucleatum depășește norma &icirc;n 20 de probe și T. forsythia &icirc;n 18. F. nucleatum se găsește &icirc;n concentrații mari la majoritatea fumătorilor și doar la un pacient din categoria &bdquo;Nefumători&rdquo;. Probele cu concentrații anormale de A. actinomycetemcomitans prezintă concentrații mari pentru majoritatea speciilor. De asemenea, integrarea cu datele clinice indică prezența patologică a P. endodontalis la toți pacienții cu carcinom laringian și cca. la 30% dintre pacienții cu carcinom al buzei și orofaringelui. Concluzii. Din toate cele 7 specii studiate, F. nucleatum și T. forsythia au concentrații patologice &icirc;n majoritatea probelor.</p></description>
</descriptions>
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