Bacteria are unique organisms which represent a large and diverse group of species from varied climatic extremities, play a key role in biogeochemical processes, have a vast array of metabolic functions with quick response to any change in environmental conditions. A lot of research is inspired by isolation of novel aquatic microbes with valuable compounds and it studying by polymerase chain reaction (PCR)-amplified nucleic acids without culturing, mor-phology, physiology or ecology study. But genetic identification in complex with traditional methods allow microbial communities to be studied with greater precision.