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SM ISO690:2012 BELOUSOVA, Galina, MOGÎLDA, Anatolii. Molecular identification of Fusarium spp. Pathogens in sesame seeds. In: Biotehnologii avansate – realizări şi perspective, Ed. 6, 3-4 octombrie 2022, Chişinău. Chișinău, Republica Moldova: Centrul Editorial-Poligrafic al USM, 2022, Editia a VI-a, pp. 10-11. ISBN 978-9975-159-81-4. DOI: https://doi.org/10.53040/abap6.2022.02 |
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Biotehnologii avansate – realizări şi perspective Editia a VI-a, 2022 |
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Simpozionul "Biotehnologii avansate – realizări şi perspective" 6, Chişinău, Moldova, 3-4 octombrie 2022 | |||||||
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DOI: https://doi.org/10.53040/abap6.2022.02 | |||||||
CZU: 632.4:631.53.02:633.853.73 | |||||||
Pag. 10-11 | |||||||
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Sesame (Sesamum indicum L.) is one of the oldest oilseed crops cultivated worldwide due to its unique seed qualities. About 65% of the world sesame yield is used for cooking oil production. Sesame seeds are rich in proteins, sulphur, amino acids, minerals and vitamins. Genus Sesamum L. belongs to the plant family Pedaliaceae Lindl., which comprises about 38 species. The only domesticated species Sesamum indicum L. (2n = 26) is cultivated in many states in tropical and temperate regions. Sesamum indicum L. is considered to originate from South Africa. Fungal pathogens are associated with sesame yield losses. The fungal disease fusariosis is considered a significant threat. The causal agents are associated with root system and transmitted via soil, blocking the xylem elements. The first case of fusariosis was reported in 1950 in USA [1]. The causal agents induce cell destruction and block the vessel elements with gum, which sub-sequently detains water and plant alimentation. This further induces plant withering and death [2]. Recently, as a result of sesame’s genome sequencing and developing different molecular assays, marker assisted selection for such agronomical traits like seed yield, high oil content and quality, drought tolerance, waterlogging tolerance, disease resistance became possible. The fungus usually affects the roots causing chlorosis, stem necrosis and defoliation. As a result, mature plants wither completely [3]. Therefore, seed screening for fungal pathogens is an im-portant step in preventing disease dissemination and helps increasing crop productivity. The purpose of this study was identifying the Fusarium spp. infection in twelve sesame genotypes (Biolsadovski, Zaltsadovski, Lider, Manchurskii uluchshenyi, Kubanets 57, Donskoi belosemi-annyi, Liana, Natasha, Margo, Solnechnyi, Gusar, Serebristyi) using molecular methods. |
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Cuvinte-cheie Sesamum indicum L., Fusarium spp., seeds, nested PCR |
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