Among the most common chemicals used in household and industrial applications are surfactants. Due to widespread use surfactants are often found in the domestic and industrial wastewater as these may not be completely utilized in the application. Obviously, part of surfactant discharged could be degraded naturally. However, biodegradation alone is not sufficient when the surfactant is present in large concentration. The surfactants have an impact of the humans if these enter into the food chain. Their presence can also affect the treatment efficacy of the wastewater treatment plants. The surfactants are widely used in detergent, shampoos and cosmetics. Surfactants increase solubility of organic compounds in water and hence presence of surfactants can lead to enhancement in carcinogenic potential and dermatitis as reported in literature [1]. Considering environmental and health impacts, permissible limits for surfactants is 1 mg/L in water intended for domestic purposes and still low at 0.5 mg/L for potable water [2]. Adsorptive separation of commercial sodium benzenesulfonate (SBS) and sodium dodecylbenzenesulfonate (SDBS) was investigated in the present work using activated carbon obtaining from wood charcoal by different methods. In the case of activated carbons from wood charcoal obtained by the fluidized layer method (CAML-SF) more effective results were obtained than in case of charcoals obtained by the classical method (CAML-MC). The samples were stirred at the speed of rotation 150 rpm, amplitude 4 and the temperature of 298 K. Kinetic study yields important information about the rate of removal and time required to achieve equilibrium. Rapid adsorption rate of SDBS from solution on both CAML-SF and CAML-MC samples was observed in first 15 min which decreased and became negligible after 120 min. Around 91% SDBS removal was achieved in initial 15 min from solutions having an initial concentration as 0.5 mmol/L on CAML-SF samples which continued to 97 % at 240 min. In the case of CAML-MC samples approximately 51 % were removed in the first 15 minutes and respectively until 62 % at 240 min. The effect of temperature conditions on extent of SBS removal has been studies. Adsorption isotherms of SBS at different temperatures namely: 298 K, 308 K and 318 K were measured. Initially stock solutions of SBS and SDBS having 5 mmol/L were prepared. The required solutions having different initial concentration for experiments were obtained after appropriate dilution. BET surface area of adsorbents and the pore size distribution have been analyzed based on the Brunauer-Emmett-Teller (BET) method using the Autosorb-1 instrument based on N2 adsorption-desorption.