It is well known that many thiosemicarbazide derivatives are widely used in medicine as anticancer, antimicrobial, antituberculosis, antifungal and other drugs. In many cases, the biological activity of these substances is consistent with their structure. In this regard, the synthesis and study of the biological properties of new thiosemicarbazide derivatives is of both scientific and practical interest. The aim of the given work is the synthesis and study of the anticancer properties of N,N'-[4,4'-(perfluoro-1,4- phenylene)-bis(oxy)-bis(4,1-phenylene)]-bis[2-(pyridine-2-ylmethylene)-hydrazine-carbothioamide]. The synthesis of the final substance has been carried according to the following scheme: O O F F F F H2N NH2 + (H3C)2N-C-S-S-C-N(CH3)2 S S - S, -H2S O O F F F F SCN NCS + H2N-NH2 O O F F F F H2NNHCNH NHCNHNH2 S S N + 2 OCH - 2 H2O F O F F F O NH C S C NH S HC N HN NH N CH N N Many thiosemicarbazide derivatives selectively inhibit the growth of malignant tumors. In this connection the anticancer activity of N,N'-[4,4'-(perfluoro-1,4-phenylene)-bis(oxy)-bis(4,1-phenylene)]-bis[2-(pyridine-2- ylmethylene)-hydrazine-carbothioamide] has been studied “in vitro” on prostate cancer cells (LNCaP). The anticancer activity study has been performed according to the standard method described in [1]. It has been established that the analyzed substance inhibits the growth of this type of cells at the level of chemotherapeutic drugs used in medicine for the prevention and treatment of the corresponding type of cancer. At the concentration 10-5 mol/l the substance inhibits the growth and reproduction of 100% of cancer cells, at the concentration 10-6 mol/l the thiosemicarbazone inhibits 65,3% of cells, and at the concentration 10-7 mol/l does not display anticancer activity. The identified properties of N,N'-[4,4'-(perfluoro-1,4-phenylene)-bis(oxy)-bis(4,1-phenylene)]-bis[2-(pyridine-2- ylmethylene)-hydrazine-carbothioamide] are important for expanding the arsenal of inhibitors of growth and reproduction of LNCaP prostate cancer cells. This research was supported by the International Project 10.820.09.10/RoA.