The supercritical carbon dioxide (SC-CO2) extraction is a method that assures a high ecological level of lipid extraction using pure and harmless carbon dioxide as a solvent and excluding the possibility of environment and end product pollution [1]. In the Republic of Moldova, the annual volume of manufactured tomatoes is about 80,000 tons [2] from which remain about 3,000 tons of wastes. Nowadays, tomato wastes are used as livestock feed or as fertilizer. Nevertheless, they contain biologically active compounds: carotenoids, tocopherols, flavonoids, essential fatty acids and many others, that could be used to obtain high-quality organic products. In order to study the method of SC-CO2 extraction the fresh tomato wastes were dried from 79.0% to 5.2% of water at 42-45°C. Dried tomato wastes (DTW) contained 93.5% seed, 5.0% peel and 1.5% pulp. The highest oil yield in relation to the lipid content of DTW was 66.9%. According to the results obtained the DTW composition was as follows: 5.20% water, 21.19% lipid, 38.46% protein, 18.75% carbohydrates, 12.86% fiber, 3.5% ash, 9.60μg carotenoid. After the SC-CO2 extraction the defatted DTW consisted of: 5.36% water, 5.00% lipid, 47.03% protein, 21.73% carbohydrates, 17.86% fiber, 4.02% ash, and 3.32μg carotenoids. The fatty acid composition of SC-CO2 extract from DTW was as follows: 59.28% linoleic acid, 21.39% oleic acid, 13.69% palmitic acid, 3.00% stearic acid, 2.49% linolenic acid and 0.15% myristic acid. Thus, the unsaturated fatty acids (83.16%) are predominant, particularly polyunsaturated fatty acids (61.77%). Fig. 1. Fatty acids content in tomato seed oil Consequently, SC-CO2 extract from DTW is a good source of polyunsaturated fatty acids ω-6 and ω-3. It is also a good source of provitamin A (the carotenoid content - 421.0μg). Carotenoids are compounds that protect the body’s cells from damages which may be created by free radicals. They guard against certain types of cancer, and they fight with heart diseases by blocking the formation of LDL-cholesterol which leads to heart attacks. The lycopene is the most effective against free radicals, which has 10 times higher antioxidant activity than α-tocopherol. Several epidemiological studies show that lycopene has the property to reduce the risk of heart infarcts, the prostate and digestive tract cancer. The lycopene has a protective effect against the retinal degenerative of ocular pathologies [3]. As shown, the SC-CO2 extract and defatted DTW are good sources of biologically active compounds, which can be used to enrich food products.