Objectives. Autoimmune hepatitis (AIH) is a complex disease that represents a challenge in diagnosis and management due to the clinical, laboratory and histological heterogeneity. The accurate diagnosis of autoimmune hepatitis demands evaluation of circulating autoantibodies. Our purpose was to assess the spectrum of autoantibodies in AIH patients. Materials and methods. The study included all patients discharged from a tertiary centre of the Republic of Moldova with the diagnosis of AIH between January 2014 and February 2019. The workup involved to pick up and analyse the data from patients medical records. A total of 26 AIH patients were evaluated for the autoantibody profile. Liver biopsy was performed in all these patients. Results. In this study 24 patients with AIH were female (92,3%) and the mean age was 45.15±15.36 years. Twenty-one patients (80,8%) had type I of AIH. One single patient was diagnosed with type 2 of AIH and it presented both markers anti-liver kidney microsomal type 1 (antiLKM1) and anti-liver cytosol type 1 (anti-LC1) positive. Conventional autoantibodies were absent in 4 patients and they were diagnosed with seronegative variant of AIH basing on biochemical and histological characteristics. Anti-nuclear antibodies (ANA) were present in 17 of patients with AIH type 1 (80,95%). ANA-positive patients displayed lower levels of immunoglobulin G than those with negative ANA. Antimitochondrial antibodies (AMA) were positive in 4 (15,4%) patients and in each case were associated with ANA. Antidoublestranded DNA antibodies were positive in 10 (47,6%) of 21 patients with AIH in whom this marker was recorded and it was predominantly joined with positive ANA. Other autoantibodies like anti-smooth muscle (ASMA) were found positive in 3 patients, anti-soluble liver antigen (anti-SLA) were not found in our patients and anti-Ro52 were found in 1 patient. Conclusions. In AIH type 1 the main circulating autoantibodies are ANA and these antibodies seem not to correlate with the marker of disease severity - immunoglobulin G. The spectrum of autoantibodies in AIH is wide and their diagnostic value vary, so a careful consideration of all serological markers is necessary within the clinical, biochemical and histological findings. Key words: autoimmune hepatitis, antibodies, antinuclear antibodies, anti liver kidney microsome antibodies. References 1. C. P. Guidelines, “Guidelines Easl Autoimmune ,” vol. 63, 2015. 2. Abdulrahman A. et al., “The Saudi Association for the Study of Liver Diseases and Transplantation clinical practice guidelines for management of autoimmune hepatitis”, Saudi J Gastroenterol. 2018 Nov; 24(Suppl 1): S1–S20.