Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
1027 12 |
Ultima descărcare din IBN: 2023-07-16 18:03 |
SM ISO690:2012 CASIAN, Igor, LUCA, Victor, UNGUREANU, Ion, CASIAN, Ana. Optimizarea metodeiHPLC pentru analiza fructelor de armurariu (silybum marianum (l.) gaertn. In: Analele Ştiinţifice ale USMF „N. Testemiţanu”, 2011, nr. 1(12), pp. 409-414. ISSN 1857-1719. |
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Analele Ştiinţifice ale USMF „N. Testemiţanu” | ||||||
Numărul 1(12) / 2011 / ISSN 1857-1719 | ||||||
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Pag. 409-414 | ||||||
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Rezumat | ||||||
Optimization of the HPLC method for analysis of Milk-Thistle fruit (Silybum marianum (L.)
Gaertn).
An interlaboratory irreproducibility of the results of Milk-Thistle fruit assay has been
discovered at realization of analytical procedure by the European Pharmacopoeia. It was found,
that this irreproducibility is caused by insufficient plenitude of the analytes extraction from
vegetal material.
A new procedure of probe preparation, that consists of two sequential extractions by ethylic
alcohol 40% for 60 min at 70°С, has been developed. The offered procedure ensures recovery
about 98 % and essentially decreases the volume of work.
Use of acetonitrile in the mobile phase composition and optimization of the gradient shape
or switch-over to isocratic elution are offered for decrease of the analysis time. |
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Cuvinte-cheie Silybum marianum, flavonolignans, HPLC-UV, pharmaceutical analysis. |
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