The protection of nature, including the conservation of habitats and species of community importance, is a fundamental objective for the European Union. Reducing landscape fragmentation and improving landscape connectivity are important necessities, both at European Union and global scale. The new EU’s Biodiversity Strategy for 2030, the European Green Deal and The 8th Action Program for Environment (2021-2030) highlight nature conservation and reducing ecosystem degradation, member states having to implement complex policies and programs to attain the established targets. Maintaining and enhancing ecological connectivity among and between protected areas network, must be one of the priorities for the biodiversity conservation in Romania, given the ample benefits this would provide. Consequently, a range of measures for reducing landscape fragmentation and increasing landscape connectivity have to be integrated into the Romanian protected areas management plans. This study will demonstrate this necessity and will present a series of methods and instruments for evaluating the landscape fragmentation and connectivity, including protected areas connectivity relevant for species. It should be the basis on which priority measures should be designed and included in the protected areas management plans in the present and in the future. We consider the proposed analysis as an important instrument to improving the management of protected areas and the conservation status for habitats and species.