This paper presents the results of obtaining optically active spiro- [cyclopropane-oxindoles] and studying their anti-HIV activity. Following the testing of many synthesis methods, conditions have been proposed that have allowed to obtain the target compounds with enantioselectivity more than 95% ee. Diastereoselective separation of enantiomers was also performed for some derivatives. The ability of synthesized compounds to inhibit the action of the tobacco mosaic virus- TMV, tomato spotted wilt virus-TSWV and cucumber mosaic virus-CMV has been studied and it has been shown that the hit substances are active at concentrations 0.25%. Keywords: synthesis, spiro[cyclopropane-oxinole], cytotoxicity, antiviral activity, stereoselectivity, diastereoseparation, tobacco mosaic virus, tomato spotted wilt virus, cucumber mosaic virus.