Articolul precedent |
Articolul urmator |
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Ultima descărcare din IBN: 2023-11-18 23:23 |
SM ISO690:2012 STOIAN, Alina, PAVLOVSCHI, Elena, NACU, Viorel, SANJA, Sladic, BIRGIT, Andree, HILFIKER, Andres. Principles of decellularization for composite vascularized bone graft. In: Cercetarea în biomedicină și sănătate: calitate, excelență și performanță, Ed. 1, 20-22 octombrie 2021, Chişinău. Chișinău, Republica Moldova: 2021, p. 337. ISBN 978-9975-82-223-7 (PDF).. |
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Cercetarea în biomedicină și sănătate: calitate, excelență și performanță 2021 | ||
Conferința "Cercetarea în biomedicină și sănătate: calitate, excelență și performanță" 1, Chişinău, Moldova, 20-22 octombrie 2021 | ||
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Pag. 337-337 | ||
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Rezumat | ||
Background. By using extracellular matrices (nathural or synthetic), tissue engineering has a final aims to create autologous grafts for the effective use of replacement therapy of the organs or body segments in the absence of an immune response. Objective of the study. Analysis of the decellularization protocol of the composite (vascularized) bone graft in order to obtain a non-immunogenic vascularized extracellular bone matrix. Material and Methods. The effectiveness of the decellularization protocol was checked on 3 vascular grafts of different diameters (large, medium, small) and on bone blocks (cortical and spongy), both of porcine origin. The processed grafts were examined histologically and analyzed for the amount of DNA. The biocompatibility of the grafts was also tested. Results. The decellularization protocol used has been shown to be effective on vascular grafts with different diameters and on cortical and spongy bone blocks. Histological examination showed cell death after graft processing. DNA quantification has shown a decrease in the amount of DNA in bone grafts and the biocompatibility test has demonstrated the biocompatibility of vascular and bone grafts after processing. Conclusion. Even if soft and hard tissues are different histological structures, the decellularization protocol can be adapted for both tissue types in such a way that decellularization of composite grafts can become possible. |
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Cuvinte-cheie extracellular matrix, bone allograft, reconstructive surgery, matrice extracelulară, alogrefă osoasă, chirurgia reconstructivă |
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