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SM ISO690:2012 COJOCARI, Daniela. In vitro antibacterial activity of some plant extracts against L. Monocytogenes. In: Perspectivele şi Problemele Integrării în Spaţiul European al Cercetării şi Educaţiei, Ed. 8, 4 iunie 2021, Cahul. Cahul: Universitatea de Stat „Bogdan Petriceicu Hasdeu‖ din Cahul, 2021, Vol.8, Partea 1, pp. 291-292. ISBN 2587-3563. ISSN E-ISSN 2587-3571. |
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Perspectivele şi Problemele Integrării în Spaţiul European al Cercetării şi Educaţiei Vol.8, Partea 1, 2021 |
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Conferința "Perspectivele şi Problemele Integrării în Spaţiul European al Cercetării şi Educaţiei" 8, Cahul, Moldova, 4 iunie 2021 | |||||||
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Foodborne illness is a common, costly, life-threatening disease - but largely preventable. Researchers have identified more than 250 foodborne illnesses. Most of them are infections, caused by a variety of bacteria, viruses and parasites. Listeriosis is an alarming infection usually caused by eating food contaminated with the bacterium Listeria monocytogenes. An estimated 1,600 people get listeriosis each year, and about 260 die. In past outbreaks, foods involved included ready-to-eat meat products, such as frankfurters, meat spread (paté), smoked salmon and fermented raw meat sausages, as well as dairy products (including soft cheeses, unpasteurized milk and ice cream) and prepared salads (including coleslaw and bean sprouts) as well as fresh vegetables and fruits [1]. L. monocytogenes is a facultative Gram positive intracellular bacterium. The bacterium is able to grow at low temperatures (in refrigerators) and can multiply at low temperatures (+ 4 ° C + 6 ° C). The main risk factors for L. monocytogenes contamination and individuals at increased risk are: - consumption of raw or uncooked food; - poor hygiene in the various stages of the food chain (production, preparation, etc.) ; - Persons with cancer, diabetes, kidney, or gastrointestinal disease; - Individuals with HIV/AIDS; - Persons who take glucocorticosteroid medications and with weakened immune systems; Due to rapid development of microbial resistance against chemotherapeutic agents (mostly antibiotics), it has become essential currently to screen effective, safe, cheap, and available therapeutics from various medicinal plants—like berries—for their potential antimicrobial effect. It is known that some bioactive substances, such as polyphenols and carotenoids, may show inhibitory effects on pathogenic bacteria, including L. monocytogenes. . The aim of this study was to screen and determine significant antibacterial activity of some plant extracts in vitro on L. monocytogenes (ATCC 19118). To establish the antibacterial activity of plants extracts, the following in vitro methods were used: diffusimetric method (qualitative), successive dilutions (quantitative) and the spectrophotometric method. The following extracts were used: sea buckthorn, aronia, grapes, rosehip, hawthorn. According to the obtained data, we noticed that sea buckthorn has the most pronounced effect on Listeria, the diameter of the growth inhibition zone is 22.5 mm (well diffusion method). Identification of minimum inhibitory concentration (MIC) and minimum bactericidal concentration(MBC) was performed by double fold dilution. The minimum inhibitory concentration of sea buckthorn for L. monocytogenes was 62.5 mg/ml. Then the MIC was determined using a spectrophotometer, the OD is measured at λ = 600nm. Anything above 0.1 at OD is considered microbial growth. The "Tecon" spectrophotometer was used. In concluzion we can say that increasing the resistance of microorganisms to chemicals and conventional drugs is a serious and obvious problem worldwide. Plants and their derivatives contain a wide variety of secondary metabolites that can inhibit or slow the growth of bacteria, yeasts and molds. |
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Cuvinte-cheie Activitatea antibacterială in vitro a unor extracte de plante împotriva l., Monocytogenes, L. monocytogenes, efect antibacterian, metodă difusimetrică, extract de plante |
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