As a result of palynological, cytogenetic and scanning electron microscopy examinations, eight types of pollen sterility in the vine have been identified related to the reasons for their occurrence: genetic; chromosomal; meiotic; genomic; chromo-somal-genetic; chromosomal-genomic; polyploid and total. In the distant hybrids of F₃-F₅ generations, all genotypic variants of pollen sterility are observed – from partially fertile to semi-fertile and form with normally restored fertility and vitality. As the number of generations increases, the number of self-fertile forms increases and the number of sterile ones decreases sig-nificantly. A DRX-M₃-2 seedling with chromosomal sterility of the two gametophytes was found in F₃, in which no grains or seeds were formed, regardless of the pollination method. By crossing distant hybrids of F₃ with 2n=38 with S.V.-12-309 and S.V.-20-366, F₄ generation was obtained, in which DRX-M₄-501,502,503, etc., are fruiting, partially fruiting and infertile with 2n=38, 39. From the crossing of the distant hybrids of F₄ – DRX-M₄-520, DRX-M₄-510 (2n=38) with the cultivars GM-325-58, Cristal and Moldova, F₅ generation was created, in a population of which four artificially created species carrying the new vine genome with n=19, 2n=38, which includes the chromosomes of Vitis vinifera and V. rotundifolia.