The aim of the research was to evaluate the effectiveness of the use of nanomaterials in the culture medium of thawed cow oocytes on the viability and further development of embryos in vitro. Biotechnological, cryobiological, morphological and cytogenetic methods were used during the research, as well as methods of statistical data processing. The quality of thawed cow oocytes, which were cultivated with the addition of various concentrations of highly dispersed silica with sucrose (UFS/sucrose), was assessed to study the effect of nanomaterials on the formation of embryos in vitro. For maturation, oocytecumulus complexes of cows, which were frozen by vitrification and stored in liquid nitrogen, were used. After thawing and withdrawal of cryoprotectors, oocytes were divided into 4 groups: group A, 0.1% UFS/sucrose was added; group B - 0.01%; group C - 0.001%; group K was the control (without the addition of UFS/sucrose). It was found that the addition of UFS/sucrose at a concentration of 0.001% to the culture medium is more favorable for the further development of frozen-thawed cow oocytes and the formation of embryos in vitro (33.3%, 29 out of 87 inseminated oocytes). When studying the dynamics of cleavage of 2-cell embryos of all studied groups, it was found that the highest cleavage coefficient of embryos was 59.3% in experimental group A and the lowest 40.8% in experimental group С. The cleavage index of 3-4-cell embryos was higher in the experimental group B by 8.7% than in the control (70.6%), which confirms the positive effect of UFS/sucrose (0.001% concentration, group B).The viability of frozen-thawed oocyte-cumulus complexes of cows depends on the composition of the culture medium, which ensures more efficient formation and development of embryos outside the body. The use of 0.001% concentration of UFS/sucrose in the medium for in vitro maturation of frozen-thawed oocyte-cumulus complexes of cows leads to an increase of 12.4-16.6% in the number of obtained embryos after in vitro fertilization of thawed and matured eggs.