Taking into account the relevance of the use of inbreeding in dairy cattle breeding due to the limited number of bulls in each breed, we set a goal to establish the effectiveness of using inbred bulls of the Ukrainian Black-and-White Dairy breed to increase the productivity of their daughters, as well as preserve the genealogical structure of the domestic breed. It is proved that. the highest milk yield – 5926 kg, had cows, daughters of distantly inbred bulls, which is higher than the indicators of outbred bulls by 1338 kg and higher degrees of inbreeding (moderate and close) – by 316–367 kg. Studies have not established a significant difference between the content of fat and protein in the milk of the daughters of the studied bulls, although a larger amount of milk fat and protein was obtained from the offspring of inbred bulls. The influence of the degree of inbreeding of bulls on the milk yield of their daughters was 8.2%, on fat-milk content – 1.3% and protein-milk content – 3.0%. A conclusion was made about a positive effect on the increase in milk productivity of daughters of Ukrainian Black-and-White Dairy bulls, the inbreeding coefficient of which is 0.39–7.03%. The use of these animals will allow preserving the domestic breed and stabilizing its genealogical structure.