The purpose of this research was to reveal the effective methods of directed (artificial) queens rearing depending on organization way of the nest in cell starter colonies. The study object were the honeybee colonies from the experimental apiary of the Institute of Zoology of the Academy of Sciences of Moldova. To reveal the efficient methods of organizing the cell starter colonies nest for the queens rearing, a special experiment was carried out with 4 similar batches of bee families, with 5 families in each batch, which were different by way of nest structure. The bee families of the I batch served as a control and their nest was organized according to the standard method: in the queenless nest, populated in abundance by bees, 2 food combs (honey+bee bread) and the queen-cells bar frame with 55-65 grafted larvae at the age of 1 day, depending on the rearing series. In addition to the food combs and the queen-cells bar frame, 2 brood combs of all ages in the starter colonies of the the batch II were introduced, 2 sealed brood combs were introduces in the starter colonies of batch III and in the starter families of batch IV - 2 unsealed brood combs were added. The analysis of the research results allowed us to conclude that the presence of the brood in the combs introduced into the cell starter families has a beneficial effect on the final efficiency on the directed queens rearing. The introduction into the cell starter colonies of brood combs of all ages contributes to the obvious increase of the larval acceptance level of 2.5 times (td=25.5; P<0.001) and of the yield of obtained queens by 2.9 times (td=28.6; P<0.001), compared to the colonies in the control batch, where no brood combs were introduced. The introduction into the cell starter colonies of unsealed brood combs contributes to the obvious increase of the larval acceptance level of 2.3 times (td=20.7; P<0.001) and of the yield of obtained queens by 2.7 times (td=30.6; P<0.001), compared to the colonies in the control batch, where no brood combs were introduced. The introduction into the cell starter colonies only of the sealed brood combs contributes to no significant growth of the larval acceptance level by 4.3% (td=1.8; P<0.1) and of the yield of obtained queens by 5,3% (td=3.0; P<0.01), compared to the colonies in the control batch, where no brood combs were introduced.