Quantitative and qualitative indicators of sperm obtained from rams Moldavian Karakul were studied. The average volume of the ejaculate was 0.84 ml, 91% mobility and 2.5 mlrd / ml concentration. The action of the BD-1 preparation synthesized at the Institute of Microbiology and Biotechnology of the Academy of Sciences of the Republic of Moldova was experimented as an additional component introduced into the basic environment regarding the quality of the ram seminal material, preserved at 2-4°C. It has been found that the introduction in the composition of the basic medium for 6% dilution of seminal material of BD-1 has a positive influence on the preservation of the semen. After 144 hours of incubation at 2-4°C the sperm mobility was 68% compared to the control group where this index was 45%. Artificial insemination of the sheep with refrigerated sperm after 144 hours allowed 71.9% sheep fecundity. The proposed technology for conserving the semen of ram at 2-4°C which composition proposes the dilution medium for semen, containing: glucose, sodium citrate, egg yolk, BD-1, allows the results of artificial insemination of sheep to be made more efficient.