Breeding indices and their interrelations were studied with the Dutch and German Holstein cow populations, exploited in the southern part of the Republic of Moldova. It was established that regardless of origins of cow population both duration of service-period as well as during of calving interval significantly prevail admitted optimal value. The best results regarding the time of service -period and interval between calving is registered at the population of Holstein cows of Dutch origin. The calving interval, in the average on population of cows of Dutch origin exceeds the optimal admissible value by 16.93%. Under similar conditions, maintenance and operating on cows of the German origins the calving interval duration exceeds the target value by 21.2%, being higher comparing with the results established at cows of Dutch origins by 3.5%. The coefficient of reproductive capacity utilization on average at cows in cows of Dutch origin is 0.86 and that of German origin is 0.83. In the investigated populations, were established links under medium, weak and very weak, both positive and negative, between breeding indices and the age of cows.